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KMID : 1161420110140070767
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Journal of Medicinal Food
2011 Volume.14 No. 7 p.767 ~ p.774
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Antinociceptive, Free Radical?Scavenging, and Cytotoxic Activities of Acanthus hirsutus Boiss
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Harput Ummuhan Sebnem
Arihan Okan
Iskit Alper B.
Nagatsu Akito
Saracoglu Iclal
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Abstract
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In vivo antinociceptive and in vitro radical scavenging and cytotoxic activities of Acanthus hirsutus Boiss. aqueous extract were investigated to give a new insight into plant usage in traditional medicine. The extract showed significant antinociceptive activity in acetic acid?induced writhing test in mice after oral application and did not change the hind-leg retraction period in the hot-plate test for any dose applied. In addition, the extract showed radical-scavenging activity against 2,2-diphenyl-1-picryl-hydrazyl, nitric oxide, and superoxide radicals similar to those of standard compounds 3-t-butyl-4-hydroxyanisole, ascorbic acid (vitamin C), and quercetin. The gallic acid equivalent total phenolic content of the plant was found to be 65.4?mg/g dry extract. Cytotoxic activity of the aqueous extract was tested against 3 different cancer cell lines?Hep-2 (human larynx epidermoid carcinoma), RD (human rhabdomyosarcoma), and L20B (transgenic murine L cells)?and 1 noncancerous cell line (VERO) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Through the phytochemical studies, the following compounds were isolated: 3 lignan glucosides [(?)-syringaresinol-di-O-β-glucopyranoside, (?)-medioresinol-di-O-β-D-glucopyranoside, (?)-pinoresinol-4′-O-β-glucopyranoside], 2 benzoxazinoids [2-hydroxy-1,4-benzoxazin-3(4H)-one, (2R)-2-O-β-glucopyranosyl-1,4-benzoxazin-3(4H)-one], 4 phenylethanoid glycosides (acteoside, leucosceptoside A, martynoside, hattushoside), and 2 phenylpropanoid glucosides (sinapyl aldehyde-4-O-β-glucopyranoside, sinapyl alcohol-4-O-β-glucopyranoside). Cytotoxic and radical-scavenging activities of the isolated compounds were also determined. 2-hydroxy-1,4-benzoxazin-3(4H)-one and acteoside were the most active compounds in both experiments.
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KEYWORD
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Acanthus, antinociceptive effect, cytotoxicity, radical scavenging effect
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